Primer
Part:BBa_K268019:Design
Designed by: Nora Yucel Group: iGEM09_UChicago (2009-10-21)
YOR186W -> GFP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2463
Illegal PstI site found at 1227 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2463
Illegal PstI site found at 1227 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2463
Illegal BglII site found at 31
Illegal BglII site found at 1017
Illegal BamHI site found at 46 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2463
Illegal PstI site found at 1227 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2463
Illegal PstI site found at 1227 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 706
Design Notes
Source
Promoter region from gene YGR035C in S. cerevisiae as obtained through yeastgenome.org. Gene deletion and tagging cassette from Longtine, et al (1998).